Quantifying Reversible Surface Binding via Surface-Integrated Fluorescence Correlation Spectroscopy

An integrated platform for surface forces measurements and fluorescence correlation spectroscopy

We describe an apparatus to measure the diffusion of dilute fluorophores in molecularly thin liquid films within a surface forces apparatus ~SFA!. The design is a significant modification of the traditional SFA in that it allows one to combine nanorheology with the single-molecule sensitive technique of fluorescence correlation spectroscopy. The primary enabling idea was to place a miniaturized...

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

This article focuses on methods based on fluctuation correlation spectroscopy to determine the formation of protein complexes in living cells. We present the principles of the fluctuation method applied to cells. We discuss the novelty and the promises of this approach. The emphasis is in the discussion of the underlying statistical assumptions of the image correlation spectroscopy analysis rat...

Analysis of Ligand-Receptor Interaction on the Surface of Living Cells by Fluorescence Correlation Spectroscopy

Ligand-receptor interaction on the cell surface has traditionally been analyzed by using radioisotope-labeled ligands. The reaction is carried out at low temperatures such as 4C or on ice in order to prevent endocytosis of the ligandreceptor complex, followed by extensive washing to remove unbound ligands from cells. To overcome these drawbacks, in the present study we have employed fluorescenc...

Screening for FtsZ Dimerization Inhibitors Using Fluorescence Cross-Correlation Spectroscopy and Surface Resonance Plasmon Analysis

FtsZ is an attractive target for antibiotic research because it is an essential bacterial cell division protein that polymerizes in a GTP-dependent manner. To find the seed chemical structure, we established a high-throughput, quantitative screening method combining fluorescence cross-correlation spectroscopy (FCCS) and surface plasmon resonance (SPR). As a new concept for the application of FC...

Fluorescence correlation spectroscopy.